Tie of the round sees Newcastle & District Premier Division's Ballyvea host St. Luke's at the Carginagh Road Playing Fields in Kilkeel.
St. Luke's, from the Northern Amateur League, have been involved in the tie of the round once already this season, a 2-0 defeat of the highly rated Hill Street back in Round Four.
Ballyvea come into the game on the back of victory over BBOB Londonderry in the last round while, prior to that, BBOB Portadown, Ambassadors and Magheraveeley all fell by the wayside.
The Kilkeel-based outfit have only played a handful of league games this season but have been battling on a number of fronts in the many cup competitions available to them.
As well as their Junior Cup commitments, they are also into the last eight of the Junior Cup and collected runners-up medals in the Bobby Dalzell Cup.
Junior Cup holders, Harryville Homers, continue their defence at the Wakehurst Playing Fields in Ballymena when Belfast & District Premier Division side Cumann Spoirt an Phobail visit.
The Homers are gunning for a third successive title and the sides they defeated in the previous two finals, Rosemount Rec. and Lisbellaw United, meet in Fermanagh.
The other last eight game sees Killen Rangers host Ballymena's Carniny Rangers.
Irish Junior Cup - Quarter-finals - 27th February (kick-off 1:30pm)
Ballyvea v. St. Luke's - Carginagh Road Playing Fields, Kilkeel
Harryville Homers v. Cumann Spoirt an Phobail - Wakehurst Playing Fields, Ballymena
Killen Rangers v. Carniny Rangers - Killen Village
Lisbellaw United v. Rosemount Rec. - Drumad, Lisbellaw